P-216: The Protective Effect of Saturejakhuzistanicaagainst Female Mice Follicle Defects Induced by Busulfan

Background: In the past 2 decades, the survival rates for many of the malignancies that affectyoung women have significantly improved. For many of these malignancies, survival rates exceed 80-90%. Therefore, the remote effects of cancer treatment have recently gained worldwide interest, andthe protection against iatrogenic infertility caused by chemotherapy assumes high priority. Busulfan is a bifunctional alkylating agent.This produces reactive carbonium ions that can alkylate DNA and effects on ovary.. In this study we use extract of saturejakhuzistanicaas protective agent for restoration follicles againstthe side effects ofbusulfan on ovarian follicles. Materials and Methods: 48 adult female mice were randomly divided into 6 groups (n=8): control group received only vehicle orally once a day,two chemotrapy groups were gavaged and injected (ip) with Busulfan,a group were gavaged with saturejakhuzistanica,and another two groups were gavaged and injected (ip) with Busulfan andweregavaged with saturejakhuzistanica.after 35 days we prepare cross section of ovarian tissues. the tissue section were stained with HandE and then consider underlight microscope observation.the number of different stage of growing and corpus loteumswere counted and then in this experiment the rate of healthy and atretic follicles were evaluated. Results: The results obtained from this study showed thattreated mouse with busulfan can reduce thenumber of follicles and increased percent of atreticfollicles,Administration of saturejakhuzistanica in group 5 and 6 significantly restored the primordial follicles the deterioration of follicles by busulfan and improved quality and number of follicles in different stage of growth (p<0.05). Conclusion: the present study indicates that administration of busulfan decrease number and quality of follicles and saturejakhuzistanica may have protective effect against busulfan defect.